Premium
Quantitative detection of volatile sulfur compound‐ producing microorganisms in oral specimens using real‐time PCR
Author(s) -
Kato H,
Yoshida A,
Awano S,
Ansai T,
Takehara T
Publication year - 2005
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2005.01096.x
Subject(s) - tannerella forsythia , treponema denticola , fusobacterium nucleatum , taqman , bacteria , microbiology and biotechnology , real time polymerase chain reaction , actinobacillus , forsythia , porphyromonas gingivalis , bacteroides , chemistry , biology , medicine , pathology , honeysuckle , biochemistry , alternative medicine , traditional chinese medicine , gene , genetics
Objective: It is well‐known that some periodontopathic bacteria, especially Porphyromonas gingivalis , Fusobacterium nucleatum , Tannerella forsythia (formerly Bacteroides forsythus or Tan. forsythensis ), and Treponema denticola , actively produce volatile sulfur compounds (VSCs), such as H 2 S and CH 3 SH. We previously reported a qualitative relationship between periodontopathic bacteria and VSCs; however, a quantitative analysis of periodontopathic bacteria in oral specimens is required for further characterization of the relationship between oral bacteria and VSCs. In this study, we report a real‐time polymerase chain reaction (PCR) method for the quantitative analysis of VSC‐producing bacteria in oral specimens. Subjects and methods: Specimens were collected from 22 patients who visited the Preventive Dentistry and Breath Odor Clinic of Kyushu Dental College. A real‐time PCR assay using the TaqMan system, based on the 5′–3′ exonuclease activity of Taq polymerase, was employed for the quantitative analysis of periodontopathic bacteria that produce VSCs. Results: Using real‐time PCR, we performed a quantitative analysis of P. gingivalis , F. nucleatum , Tan. forsythia , and T. denticola in the saliva, on the tongue coat, and in the subgingival plaque of patients with oral malodor. Conclusion: Real‐time PCR using the TaqMan system can be used for the quantitative analysis of VSC‐producing oral bacteria.