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Expression of integrin subunits α 2, α 3, α 5, α v, β 1, β 3 and β 4 in different histological types of ameloblastoma compared with dental germ, dental lamina and adult lining epithelium
Author(s) -
Modolo F,
Martins MT,
Loducca SVL,
De Araújo VC
Publication year - 2004
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2004.01028.x
Subject(s) - integrin , dental lamina , ameloblastoma , basal lamina , staining , pathology , epithelium , immunohistochemistry , biology , anatomy , ultrastructure , medicine , cell , maxilla , genetics , odontogenic
Objective: To analyze integrin expression and distribution in different histological types of ameloblastoma, compared with dental germ, dental lamina and adult lining epithelium. Materials and methods: Three‐micrometer sections from paraffin‐embedded specimens were evaluated employing a streptavidin–biotin immunohistochemical method and anti‐integrin α 2, α 3, α 5, α v, β 1, β 3 and β 4 antibodies. Results: All integrins were present in all specimens, exhibiting different patterns. In follicular ameloblastoma, the integrin staining was stronger in the periphery while integrin α 2 was not present in the central cells. Acanthomatous ameloblastoma showed a similar pattern, with positive staining for integrins α 3, α 5, α v, β 1 and β 4 in the metaplastic cells. In the unicystic, integrin staining was uniform except for integrins α 5 and β 3 which showed weaker staining in the upper layers. In the plexiform ameloblastoma, dental germ and lamina integrin staining was uniform. In the adult lining epithelium, staining for integrins α 2, α 5 and β 4 was confined to the basal layer, while integrins α v and β 3 were present in the basal and parabasal, with integrins α 3 and β 1 in the upper layers. Conclusion: Acanthomatous, follicular and unicystic ameloblastomas showed integrin staining patterns similar to the adult lining epithelium while the plexiform ameloblastoma was similar to the dental germ and lamina.