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Cytokine and chemokine response of bone cells after dentin challenge in vitro
Author(s) -
Silva TA,
Lara VS,
Rosa AL,
Cunha FQ
Publication year - 2004
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2004.01011.x
Subject(s) - cytokine , chemokine , chemistry , dentin , osteoblast , immunology , microbiology and biotechnology , tumor necrosis factor alpha , in vitro , inflammation , medicine , pathology , biology , biochemistry
Objective: The aim of this study was to characterize the effects of dentin extracts on cytokine, chemokine and nitric oxide (NO) production by primary rat bone cells. Study design: Osteoblastic bone marrow cultures were exposed to particulate (D‐part), non‐particulate (D‐n‐part) and demineralized dentin extracts and evaluated for proliferative activity, cell morphology, alkaline phosphatase activity and bone‐like nodule formation. Cytokine production was assessed by enzyme‐linked immunosorbent assay and NO release by the Griess method. Results: The dentin extracts did not affect osteoblast numbering. Conversely, they up regulated in a dose‐dependent manner the production by the osteoblasts of the pro‐inflammatory interleukin‐1 β (IL‐1 β ), tumor necrosis factor‐ α , IL‐6, cytokine‐induced neutrophil chemoattractant‐1, and of the anti‐inflammatory cytokine, IL‐10. The NO production was stimulated only by D‐n‐part. Conclusion: These results demonstrate that dentin induces the production of inflammatory cytokines by osteoblasts and suggest that pro‐resorptive pathways might be stimulated when dentin molecules come into contact with bone cells during pathological processes associated with dentin and bone matrix dissolution.