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Cytokine mRNA expression in the labial salivary glands of healthy volunteers
Author(s) -
Brennan MT,
Fox PC
Publication year - 2000
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2000.tb00117.x
Subject(s) - ductal cells , acinar cell , cytokine , salivary gland , biology , pathology , endocrinology , medicine , immunology , pancreas
OBJECTIVE: To examine the cytokine profile in minor salivary glands of healthy volunteers and examine differential cytokine production from acinar and ductal tissueS. STUDY DESIGN: Acinar and ductal elements were isolated by a cell‐specific micro‐dissection technique from frozen 8 μm sections of minor salivary glands from healthy volunteerS. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) and Southern hybridization using 32 P‐labeled cytokine gene‐specific probes were utilized to detect cytokine mRNA.mRNA transcripts for the Th1 cytokines (IL‐2, TNF‐alpha, and IFN‐gamma), Th2 cytokines (IL‐4, IL‐6, and IL‐10), TGF‐beta, actin, and CD3δ were analyzed in acini, ducts and total tissue sections of minor salivary glands of six healthy controls. RESULTS: Actin, TGFβ and TNFα was present in 6/6 acinar, 6/6 ductal, and 6/6 total section sampleS. IFNγ was present in 2/6 acinar, 3/6 ductal, and 6/6 total sampleS. IL‐2 was found in 3/6 acinar, 3/6 ductal and 5/6 total sectionS. IL‐6 was found in 1/6 acinar, 0/6 ductal, and 5/6 total sectionS. IL‐10 was found in 3/6 acinar, 3/6 ductal, and 5/6 total sectionS. IL‐4 was present in 0/6 acinar, 0/6 ductal and 1/6 total sampleS. CD3δ was present in 6/6 acinar, 5/6 ductal and 6/6 total sections. CONCLUSIONS: These results demonstrate abundant expression of cytokines in healthy minor salivary glands. The production of these cytokines in healthy tissues demonstrates a likely role of supporting normal cellular function.