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Cytotoxicity of methyl methacrylate (MMA) and related compounds and their interaction with dipalmitoylphosphatidylcholine (DPPC) liposomes as a model for biomembranes
Author(s) -
Fujisawa S.,
Atsumi T.,
Kadoma Y.
Publication year - 2000
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.2000.tb00116.x
Subject(s) - dipalmitoylphosphatidylcholine , cytotoxicity , methacrylate , liposome , chemistry , differential scanning calorimetry , membrane , methyl methacrylate , monomer , nuclear chemistry , organic chemistry , phospholipid , phosphatidylcholine , biochemistry , in vitro , polymer , physics , thermodynamics
OBJECTIVE: To clarify the potential mechanism of action of methyl methacrylate (MMA) and related compounds to membranes of living cells, compared with their interaction with dipalmitoylphosphatidylcholine (DPPC) liposomes as a model for biological membranes. MATERIALS AND METHODS: For (meth)acrylates, MMA, ethyl acrylate(EA), n ‐butyl acrylate (BA) and n ‐butyl methacrylate (BMA) and for living cells, primary human gingival fibroblast (HGF), human submandibular gland adenocarcinoma cell line (HSG) and human erythrocytes were used. The physicochemical changes in DPPC liposomes induced by (meth)acrylates were studied using differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). RESULTS: Cytotoxicity decreased as follows: BA>BMA>EA>MMA.Changes in phase transition properties (temperature T m , enthalpy ΔH and Height/Half‐Height Width (H/HHW) of DSC peak were decreased as follows: BA>EA>MMA. BMA enhanced H/HHW and increased T m slightly. NMR‐shielding effect decreased as follows: BMA>MMA>BA, EA. CONCLUSION: BA and BMA exhibited large cytotoxicity and high DPPC‐interaction due to their lipophilicity, compared to EA or MMA.MMA showed little cytotoxicity and small changes in DPPC liposomes, whereas BA showed large cytotoxicity and large changes in the liposomes characterized by the membrane disturbance. Hae‐molytic activity and cytotoxicity of acrylates were higher than those of methacrylates. The physico‐chemical properties (Log P or Q σ ) of (meth) acrylates affect the lipid bilayer in biological membranes.