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Clinical applications of two detection systems for HIV using saliva
Author(s) -
Wisnom C,
DePaola L,
Saville RD,
Constantine NT,
Falkler W
Publication year - 1997
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.1997.tb00382.x
Subject(s) - saliva , human immunodeficiency virus (hiv) , hiv screening , medicine , immunology , test (biology) , biology , men who have sex with men , syphilis , paleontology
The purpose of this research was to determine if the accuracy of HIV saliva and serum test results were influenced by changes in collection sites. In order to do so, serum and saliva samples were collected from 615 subjects in eight different geographic settings. The oral fluid collection/testing systems utilized were the Orapette SalivaCard HIV‐1/HIV‐2 antibody test (Trinity Biotech, Ireland) and the Omni‐SAI/Immuno Comb II HIV 1 & 2 Saliva Test (Orgenics Ltd, Israel). For comparison, serum samples were tested by ELISA (Ortho) with reactive results confirmed via HIV‐1 and HIV‐2 Western blots (Biotech/Dupont, Institute Pasteur). The HIV serum and oral fluid collections were conducted in numerous test sites, which provided a great diversity in temperature, lighting and physical layout. The tests proved to be 99.8% and 100% specific, and both were 100% sensitive, regardless of the physical setting of the collections. While these systems are not currently available in the US, this study clearly demonstrates they can accurately be utilized in a variety of clinical settings, providing great promise for future applications.