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Diurnal variation of γ‐glutamyl transpeptidase activity during DMBA‐induced hamster buccal pouch carcinogenesis
Author(s) -
Lin LM,
Chen YK
Publication year - 1997
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.1997.tb00027.x
Subject(s) - dmba , buccal administration , cheek pouch , hamster , carcinogenesis , pouch , diurnal temperature variation , medicine , chemistry , pathology , biology , endocrinology , anatomy , pharmacology , cancer , atmospheric sciences , geology
OBJECTIVE: The aim of the present study is to assess the diurnal variation of γ‐glutamyl transpeptidase (GGT) during 7,12‐dimethylbenz[a]anthracene (DMBA)‐induced hamster buccal pouch carcinogenesis. MATERIALS AND METHODS: The right buccal pouches of 108 Syrian golden hamsters, divided into three experimental groups, were treated three times weekly with 0.5% DMBA in mineral oil over an 11‐week treatment regimen. The left buccal pouches were untreated and served as the controlS. Within each group, six animals were killed at 4‐h intervals (04.00, 08.00, 12.00, 16.00, 20.00 and 24.00) for 24 h. GGT histochemical stain, according to the method of Ruthenberg and coworkers, was applied. The number of GGT‐positive foci in the pouch mucosa was recorded at 3, 7 and 11 experimental weeks. RESULT: Diurnal variation of GGT histochemical activity during DMBA‐induced hamster buccal pouch carcinogenesis was substantiated in the present study. CONCLUSION: This investigation highlights the importance of the diurnal variation in experimental oral carcinogenesis.