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TCR Vβ gene expression in lesional T lymphocyte cell lines in oral lichen planus
Author(s) -
Zhou XJ,
Savage NW,
Sugerman PB,
Walsh LJ,
Aldred MJ,
Seymour GJ
Publication year - 1996
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.1996.tb00241.x
Subject(s) - oral lichen planus , superantigen , t cell receptor , microbiology and biotechnology , antigen , polymerase chain reaction , lymphocyte , t lymphocyte , complementary dna , biology , gene , gene expression , t cell , cell culture , immunology , immune system , genetics
OBJECTIVE: To study Vβ gene expression in oral lichen planus (OLP) lesional T lymphocyte cell lines. MATERIALS AND METHODS: Lesional T lymphocytes were isolated from eight OLP patients and cell lines established. The total RNA was extracted from these lymphocyte cell lines and reverse transcribed. cDNA was amplified by the polymerase chain reaction (PCR) using a panel of 26 Vβ‐specific oligonucleotide primers followed by qualitative analysis of the electrophoresed reaction products. RESULTS: Vβ 1, 2, 3, 5.1, 6.1–3, 7, 8, 9, 22. 23, and 24 were represented consistently in all of the OLP samples, Vβ11, 12, and 17 were consistently negative, while the other Vβ families (Vβ4, 5.2–3, 10, 13.1, 13.2, 14, 15, 16, 18, 19, 20, and 21) were variable. Vβ22 and 23 were the most strongly expressed in all patients. CONCLUSIONS A limited T cell receptor (TCR) gene usage indicates a degree of oligoclonality within these lesional T lymphocyte cell lines from OLP. This implies that OLP may be an antigen‐specific disease or linked to a limited number of superantigens.