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Analysis of cultivable Porphyromonas gingivalis with trypsin‐like protease enzyme activity and serum antibodies in chronic adult periodontitis
Author(s) -
Smith AJ,
Wade WG,
Greenman J,
Addy M
Publication year - 1995
Publication title -
oral diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.953
H-Index - 87
eISSN - 1601-0825
pISSN - 1354-523X
DOI - 10.1111/j.1601-0825.1995.tb00162.x
Subject(s) - porphyromonas gingivalis , periodontitis , microbiology and biotechnology , gingivitis , chronic periodontitis , antibody , immunology , protease , antigen , medicine , enzyme , chemistry , biology , dentistry , biochemistry
OBJECTIVE: Trypsin‐like protease (TLPase) enzyme produced by Porphyromonas gingivalis has been implicated as a virulence factor in the pathogenesis of periodontal disease. The aims of this study were to investigate the relationship between cultivable P. gingivalis , TLPase enzyme activity (BANA hydrolysis) and serum antibody levels against cell sonicate and a purified TLPase antigen from P. gingivalis W50. MATERIALS AND METHODS: Sub‐gingival plaque samples were cultured for levels of P. gingivalis together with a chairside analysis of TLPase enzyme activity (Perioscan) from periodontitis and gingivitis sites of adult periodontitis patients. A TLPase from P. gingivalis was purified by gel filtration and ion exchange chromatogra‐phy from the vesicle fraction for use as a test antigen. RESULTS: Elevated levels of P. gingivalis were found at periodontitis sites, however, there was no correlation with sub‐gingival plaque TLPase enzyme activity. Adult periodontitis patients had higher levels of IgG and IgA against cell sonicate and TLPase antigens than did controls. Those patients who were P. gingivalis culture‐positive demonstrated an elevated immune response against both cell sonicate and TLPase when compared to P. gingivalis culture‐negative patients. Treatment resulted in an improvement of clinical indices and no cultivable P. gingivalis could be recovered from the treated sites and there was a concomitant decrease in IgG levels against the TLPase. There was no significant difference in BANA hydrolysis at gingivitis sites or periodontitis sites after treatment. CONCLUSIONS Further longitudinal studies are suggested to investigate the role of the TLPase in the response to treatment of chronic adult periodontitis patients.