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A multiparametric assay to compare the cytotoxicity of soy milk with different storage media
Author(s) -
Silva Emmanuel J. N. L.,
Rollemberg Carolina B.,
Souza CoutinhoFilho Tauby,
Zaia Alexandre A.
Publication year - 2013
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.2012.01175.x
Subject(s) - soy milk , crystal violet , viability assay , cytotoxicity , food science , cell counting , soy protein , chemistry , balanced salt solution , neutral red , cell , biology , biochemistry , in vitro , microbiology and biotechnology , organic chemistry , cell cycle
Background/Aim The aim of this study was to evaluate the cytotoxicity of soy milk compared with several other storage media [coconut water, Hank′s Balanced Salt Solution ( HBSS ) and whole milk], assessed through a multiparametric analysis employing 3 T 3 cells. Materials and methods Plates containing confluent 3 T 3 fibroblasts were exposed to the various media for 24 h, at 37°C with 5% CO 2 , and cell viability was evaluated by a multiparametric assay assessing sequentially, on the same cells, mitochondrial activity ( XTT ), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). Results from each test were compared by two‐way analysis of variance ( anova ). Results Statistical analysis showed that whole milk, HBSS and soy milk were the most effective media in maintaining cell viability at all tested times ( P < 0.05). The least amount of viable cells was observed when using coconut water. Conclusions This study shows that the efficacy of soy milk in maintaining the viability of 3 T 3 fibroblasts is similar to that of HBSS and milk, as shown by three different cell viability tests.