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Regenerative capability of dental pulp cells after crown fracture
Author(s) -
Shima Hidehito,
Matsuzaka Kenichi,
Kokubu Eitoyo,
Inoue Takashi
Publication year - 2013
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.2012.01134.x
Subject(s) - nestin , dentin sialophosphoprotein , bone sialoprotein , odontoblast , dental pulp stem cells , osteopontin , pulp (tooth) , osteocalcin , stem cell , dentin , chemistry , immunohistochemistry , pathology , andrology , biology , anatomy , dentistry , microbiology and biotechnology , medicine , immunology , alkaline phosphatase , neural stem cell , biochemistry , enzyme
– The purpose of this study was to evaluate the characteristics of dental pulp cells for tissue engineering derived from the fractured incisal portion of tooth crowns. Thirty Sprague‐Dawley rats were used for histological and immunohistochemical analysis of nestin protein expression and to measure levels of mRNAs encoding osteocalcin, osteopontin, bone sialoprotein (BSP), dentin sialoprotein (DSP), heat shock protein (HSP) 27, vascular endothelial growth factor (VEGF), ATP‐binding cassette transporter G2 (ABCG2), nestin, and p57 Kip2 . Odontoblasts at the incisal portion in the control group were oriented in a regular pattern, but those in the experimental group were randomly stratified. Immunohistochemically, only a few odontoblasts were positive for nestin at the incisal portion in the experimental group at 2 days. Some cells in the inner area in the control group were positive for nestin, but nestin‐positive cells in the experimental group at the incisal portion were not observed. The mRNA expression for osteogenic or odontogenic markers in the experimental group was higher than in the control group. HSP27 mRNA expression in the experimental group at 2 days was higher than in the control group and in the experimental group at 7 days. mRNA expression of stem cell markers, such as ABCG2 and nestin, in the experimental group tended to decrease compared with the control. In conclusion, this study demonstrates that dental pulp stem cells derived from fractured teeth differentiate to osteogenic or odontogenic cells.