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Histological and immunohistochemical analyses of the chronology of healing process after immediate tooth replantation in incisor rat teeth
Author(s) -
Panzarini Sônia Regina,
Okamoto Roberta,
Poi Wilson Roberto,
Sonoda Celso Koogi,
Pedrini Denise,
da Silva Paula Ervolino,
Saito Celia Tomiko Matida Hamata,
Marão Heloísa Fonseca,
Sedlacek Paulo
Publication year - 2013
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.2012.01127.x
Subject(s) - periodontal fiber , replantation , tooth replantation , ankylosis , resorption , medicine , connective tissue , dentistry , pulp (tooth) , dental alveolus , immunohistochemistry , regeneration (biology) , incisor , anatomy , pathology , biology , root resorption , microbiology and biotechnology
– Dental tissues have special characteristics, and its regenerative capacity is noteworthy. However, understanding the circumstances that lead to regeneration is challenging. In this study, the chronology of the healing process after immediate replantation of rat incisor teeth was examined by histological and immunohistochemical analyses within a 60‐day period. Thirty‐six male Wistar rats had their maxillary right incisors extracted and replanted after 15 min in saline storage. The rats were sacrificed immediately 3, 7, 15, 28, and 60 days after replantation. The histological analysis showed rupture of the periodontal ligament and formation of a blood clot, which started being replaced by a connective tissue after 3 days. At 7 days, the gingival mucosa epithelium was reinserted and areas of root resorption could be seen. At 15 days, the periodontal ligament was repaired. At 3 days, the pulp presented an absence of the odontoblast layer, which started being replaced by a connective tissue. This tissue suffered gradual calcification, filling the root canal at 28 and 60 days. The root ends were closed. The immunohistochemical analysis revealed greater expression of OP, OPG, and RANK proteins in the initial periods (0 and 3 days), while TRAP expression predominated at 28 and 60 days ( P < 0.05). In conclusion, in delayed tooth replantation, there is great new bone formation activity in the earlier periods of the repair process, while a predominance of bone resorption and remodeling is observed in the more advanced periods.