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In vitro antibacterial activity of different endodontic irrigants
Author(s) -
Poggio Claudio,
Colombo Marco,
Scribante Andrea,
Sforza Dario,
Bianchi Stefano
Publication year - 2012
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.2011.01074.x
Subject(s) - in vitro , dentistry , antibacterial activity , traditional medicine , chemistry , medicine , biology , bacteria , biochemistry , genetics
– Aim : The objective of this study was to compare in vitro the antibacterial activity of Tetraclean (mixture of doxycycline, citric acid and polypropylene glycol), Niclor 5 (5.25% sodium hypochlorite solution), Cloreximid (0.2% chlorhexidine and 0.2% cetrimide solution) and hydrogen peroxide 12 volumes on three endodontic pathogens associated with primary endodontic infections. Enterococcus faecalis , Streptococcus mutans and Staphylococcus aureus strains were selected to evaluate the antibacterial activity of endodontic irrigants by the agar disc‐diffusion test. Material and methods : Paper discs were saturated with each one of the test solutions (at room temperature and preheating at 50°C) and placed onto culture agar‐plates preadsorbed with bacterial cells and further incubated for 24 h at 37°C. The growth inhibition zones around each irrigant were recorded and compared for each bacterial strain. Kruskal–Wallis and Mann–Whitney tests were applied to compare the various groups. Results : At room temperature, Tetraclean showed significantly higher inhibition of bacteria growth than all other irrigants tested. Preheating at 50°C significantly increased growth inhibition for all the groups tested. At 50°C, hydrogen peroxide 12 volumes and Tetraclean showed significantly higher efficacy than all other irrigants tested. Conclusions : 50°C‐preheated hydrogen peroxide 12 volumes and Tetraclean showed highest inhibition of the bacterial growth.