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Evaluation, using extracted human teeth, of Ricetral as a storage medium for avulsions – an in vitro study
Author(s) -
Rajendran Priya,
Varghese Nettiyat OOmmen,
Varughese Jolly Mary,
Murugaian Elango
Publication year - 2011
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.2011.00988.x
Subject(s) - periodontal fiber , hemocytometer , balanced salt solution , trypan blue , dentistry , medicine , replantation , in vitro , chemistry , surgery , pathology , biochemistry , organic chemistry
– The prognosis of teeth replanted following avulsion is determined by the extra‐alveolar time and storage medium used. This study was undertaken to determine the efficacy of an oral rehydration solution ‘Ricetral’, in retaining the vitality of periodontal ligament cells when used as a storage medium for avulsed teeth prior to replantation. The study consisted of a comparative evaluation between Ricetral and two currently recommended solutions, Hank’s balanced salt solution (HBSS) and milk. Thirty extracted teeth were dried for 30 min and soaked in the respective storage media for 45 min. The periodontal ligament cells were isolated by an enzyme treatment with collagenase and trypsin. The cells were evaluated for vitality by trypan blue staining and number of vital cells counted in a hemocytometer. Statistical analysis revealed that cell vitality was high with Ricetral and HBSS, but poor with milk.