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Effect of eugenol on macrophage adhesion in vitro to plastic surfaces
Author(s) -
Segura J. J.,
JiménezRubio A.
Publication year - 1998
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.1998.tb00813.x
Subject(s) - eugenol , in vitro , chemistry , macrophage , adhesion , incubation , phagocytosis , immunology , andrology , medicine , biochemistry , organic chemistry
— This study was performed to investigate the in vitro effect of eugenol on substrate adherence capacity of macrophages. hlacro‐phages were obtained from Wistar rats. The adherence capacity of macrophages to the plastic surface of Eppendorf tubes was determined. Assays were canied out in these tubes for 15 min of incubation at 37°C in a humidified atmosphere of 5% CO 2 in air. Control peritoneal macrophages showed an adherence index=52.5%. Eugenol decreased the adherence index of macrophages in a dose‐dependent manner. A 1:1000 dilution of eugenol decreased the adherence index of macrophages by 16.6% (P<0.05). Lower dilutions (1:100 and 1:10) greatly decreased the adherence index by 51% and 96.2% respectively (P<0.01). Taking into account that substrate adherence is the first step in the phagocytic process of macrophages and in antigen presentation, eugenol could inhibit macrophage function and modulate immune and inflammatory reactions in dental pulp and periapical tissues.