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An enzyme histochemical study of the influence of formocresol and calcium hydroxide on the dental pulp
Author(s) -
Eronat N.,
Mallem P. J.,
Wijnbergen M.
Publication year - 1988
Publication title -
dental traumatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 81
eISSN - 1600-9657
pISSN - 1600-4469
DOI - 10.1111/j.1600-9657.1988.tb00291.x
Subject(s) - calcium hydroxide , pulpotomy , saline , pulp (tooth) , chemistry , lactate dehydrogenase , calcium , dentistry , hard tissue , hydroxide , h&e stain , physiological saline , staining , pathology , medicine , biochemistry , enzyme , inorganic chemistry , organic chemistry
Sections of goat incisor pulps were studied after a 5‐min application of formocresol (FC) or after calcium hydroxide or saline/ZnOE treatment followed by freeze‐sectioning. After 3–7 days the incisal part of the pulp was necrotic, as shown by absence of lactate dehydrogenase (LDH) activity. The necrotic zone was much larger after FC than after saline/ZnOE or calcium hydroxide. The LDH negative area after application of FC appeared to consist of a layer showing nuclei that were well‐preserved by the formaldehyde from the FC, and an area of tissue breakdown, which showed no or a reduced structure according to hematoxylin and eosin. Well‐preserved nuclei were not found in the 2 other groups. After 14 days all necrotic tissue, except that subjacent to calcium hydroxide, had been replaced by vital (LDH‐positive) tissue. After saline/ZnOE or calcium hydroxide treatment, hard‐tissue formation was found in all teeth, and after FC treatment in the majority of teeth.