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B Cells Help Alloreactive T Cells Differentiate Into Memory T Cells
Author(s) -
Ng Y.H.,
Oberbarnscheidt M. H.,
Chandramoorthy H. C. K.,
Hoffman R.,
Chalasani G.
Publication year - 2010
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2010.03223.x
Subject(s) - cytotoxic t cell , interleukin 21 , cd40 , il 2 receptor , immunology , natural killer t cell , microbiology and biotechnology , antigen presenting cell , cd8 , adoptive cell transfer , t cell , zap70 , biology , antigen , immune system , in vitro , biochemistry
B cells are recognized as effector cells in allograft rejection that are dependent upon T cell help to produce alloantibodies causing graft injury. It is not known if B cells can also help T cells differentiate into memory cells in the alloimmune response. We found that in B‐cell‐deficient hosts, differentiation of alloreactive T cells into effectors was intact whereas their development into memory T cells was impaired. To test if B cell help for T cells was required for their continued differentiation into memory T cells, activated T cells were sorted from alloimmunized mice and transferred either with or without B cells into naïve adoptive hosts. Activated T cells cotransferred with B cells gave rise to more memory T cells than those transferred without B cells and upon recall, mediated accelerated rejection of skin allografts. Cotransfer of B cells led to increased memory T cells by enhancing activated CD4 T‐cell proliferation and activated CD8 T‐cell survival. These results indicate that B cells help alloreactive T‐cell differentiation, proliferation and survival to generate optimal numbers of functional memory T cells.