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Donor Lymphoid Organs Are a Major Site of Alloreactive T‐Cell Priming Following Intestinal Transplantation
Author(s) -
Wang J.,
Dong Y.,
Sun J.Z.,
Taylor R. T.,
Guo C.,
Alegre M.L.,
Williams I. R.,
Newell K. A.
Publication year - 2006
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2006.01516.x
Subject(s) - lymphatic system , lymph node , immunology , transplantation , immune system , immunogenicity , priming (agriculture) , medicine , lymph , t cell , pathology , biology , germination , botany
We hypothesized that lymphoid organs within intestinal allografts contribute to their immunogenicity. Consistent with this hypothesis recipient T cells rapidly migrated to the lymph nodes and Peyer's patches of syngeneic and allogeneic intestinal grafts such that at 24 h approximately 50% of the lymphocytes isolated from donor lymphoid organs were of recipient origin. However, only in the lymphoid organs of allografts did recipient T cells display an activated phenotype, proliferate and produce IFNγ. Rejection of allogeneic intestines lacking lymphoid organs was dramatically impaired in splenectomized, lymph node‐deficient recipients compared to lymph node bearing, wild‐type allogeneic intestines. This demonstrates the important role of donor lymphoid organs in the rejection process. Furthermore, recipient T cells proliferated more extensively and produced more IFNγ in donor lymphoid organs than in recipient lymphoid organs, indicating that donor lymphoid organs play a dominant role in initiating the recipient anti‐donor immune response following intestinal transplantation.