Premium
Improved Long‐Term Graft Survival after HO‐1 Induction in Brain‐Dead Donors
Author(s) -
Kotsch K.,
Francuski M.,
Pascher A.,
Klemz R.,
Seifert M.,
Mittler J.,
Schumacher G.,
Buelow R.,
Volk H.D.,
Tullius S.G.,
Neuhaus P.,
Pratschke J.
Publication year - 2006
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2005.01208.x
Subject(s) - medicine , brain dead , term (time) , surgery , transplantation , physics , quantum mechanics
Brain death (BD) of the donor, a risk factor uniquely relevant for organs derived from cadaver donors, influences organ quality by induction of various inflammatory events. Consequently ischemia/reperfusion injury is deteriorated and acute and chronic rejections accelerated. Donor treatment might be an approach to improve the quality of the graft. The induction of heme oxygenase 1 (HO‐1) has been shown to exert beneficial effects in living‐donor transplantation models. Therefore, we examined the impact of donor treatment with the selective inducer of HO‐1, cobalt protoporphyrin (CoPP), on organ quality and transplant outcome in a standardized BD model in a F344→LEW kidney transplant rat model. Immediately after BD induction, donor animals were administered a single dose of CoPP (5 mg/kg) and in control groups, HO‐1 activity was blocked with zinc protoporphyrin (ZnPP, 20 mg/kg). Recipients of organs from brain‐dead donors treated with CoPP survived significantly better than those from untreated brain‐dead donors (p < 0.05) and intra‐graft analysis showed improved histology (p < 0.05). Blockade of HO‐1 with ZnPP decreased the survival rates (p < 0.05) comparable to untreated brain‐dead donors. Our results demonstrate that HO‐1 induction by one single treatment of CoPP in brain‐dead donors leads to enhanced allograft survival.