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Human CD4 + CD25 + Regulatory T Cells Suppress Anti‐Porcine Xenogeneic Responses
Author(s) -
Porter Cynthia M.,
Bloom Eda T.
Publication year - 2005
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2005.00972.x
Subject(s) - allotransplantation , il 2 receptor , xenotransplantation , immune system , immunology , secretion , cytolysis , medicine , transplantation , microbiology and biotechnology , t cell , biology , cancer research , cytotoxic t cell , in vitro , endocrinology , biochemistry
Due to the shortage of human organs, xenotransplantation is being explored as an alternative to allotransplantation, but immune rejection remains a major hurdle to its implementation. We tested the ability of human CD4 + CD25 + T cells (Treg cells) to suppress CD4 + T cell‐mediated anti‐porcine xenoresponses usingin vitroassays. Human Treg cells were hyporesponsive to porcine cell stimulation and suppressed the proliferative response of CD4 + CD25 − T cells in a dose‐dependent manner, and comparison of the allo‐ and xenoresponses indicated that more Treg cells might be required to suppress the xenogeneic response than the allogeneic response. Stimulation of CD4 + CD25 − T cells with porcine cells resulted in secretion of IFN‐γ, TNF‐α, IL‐10, IL‐6 and IL‐2, and Treg cells suppressed the secretion of these cytokines, as well as the CD4 + CD25 − T‐cell cytolytic response against porcine cells. These results suggest a potential role for Treg cells in promoting xenograft survival.