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Analyses of Peripheral Blood Mononuclear Cells in Operational Tolerance After Pediatric Living Donor Liver Transplantation
Author(s) -
Li Ying,
Koshiba Takaaki,
Yoshizawa Atsushi,
Yonekawa Yukihide,
Masuda Kosuke,
Ito Atsushi,
Ueda Mikiko,
Mori Takahide,
Kawamoto Hiroshi,
Tanaka Yoshimasa,
Sakaguchi Shimon,
Minato Nagahiro,
Wood Kathryn J.,
Tanaka Koichi
Publication year - 2004
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2004.00611.x
Subject(s) - medicine , immunosuppression , peripheral blood mononuclear cell , liver transplantation , immunology , transplantation , peripheral blood , immune tolerance , living donor liver transplantation , gastroenterology , immune system , in vitro , biochemistry , chemistry
Operational tolerance (graft acceptance in an immunosuppression (IS)‐free environment) after living‐donor liver transplantation (LDLT) could occur by our elective protocol in some patients. There is, nevertheless, no reliable parameter to monitor patients who may discontinue IS without a risk of rejection. To identify such parameters, we systemically phenotyped peripheral blood mononuclear cells from operationally tolerant patients. An increase was observed in the frequency of CD4 + CD25 high+ cells, B cells and Vδ1/Vδ2 γδT‐cells ratio in operationally tolerant patients (Gr‐tol; n = 12), compared with those from age‐matched volunteers (Gr‐vol; n = 24) or patients on IS (Gr‐IS; n = 19). The frequency of NK cells was decreased in Gr‐tol, compared with those in Gr‐IS or Gr‐vol. The frequency of NKT cells was decreased after LDLT, compared with that in Gr‐vol. Although the contribution of those subsets to the tolerant state remains elusive, the results may provide important clues for reliable indicators of tolerance after LDLT.