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Role of the Liver in Peripheral Tolerance: Induction Through Oral Antigen Feeding
Author(s) -
Li Wei,
Chou Sonja T.,
Wang Celso,
Kuhr Christian S.,
Perkins James D.
Publication year - 2004
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2004.00551.x
Subject(s) - ovalbumin , medicine , adoptive cell transfer , spleen , peripheral tolerance , immunology , immune tolerance , in vivo , transplantation , in vitro , immune system , t cell , biology , biochemistry , microbiology and biotechnology
Using a murine liver transplant model, we studied the liver's role in peripheral tolerance. Livers from BALB/c mice fed with ovalbumin (OVA) at either a low or high dose were transplanted into syngeneic recipients. Non‐fed recipients were controls. Orthotopic liver transplantation (OLTx) was followed by OVA immunization and delayed‐type hypersensitivity (DTH) challenge. The ex vivo adoptive transfer effect of liver nonparenchymal cells (NPCs) or spleen cells (SCs) from OVA‐fed mice was examined. In vitro proliferative assays and cytokine profiles were conducted on NPCs and SCs from transplant recipients.Livers from all OVA‐fed mice after 10 days transferred tolerance to OVA‐naïve mice. The time course of adoptive transfer of liver NPCs from high‐dose OVA‐fed mice transferred OVA tolerance within 24 h; low‐dose OVA‐fed mice required ≥ 4 days to transfer tolerance. The in vitro proliferative response of the NPCs to OVA revealed a decreased response in both dosage groups over the control group.Our results suggest that the liver plays an important role in inducing peripheral tolerance in a mucosal tolerance model, especially feeding high‐dose OVA.