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Protection of Reduced‐Size Liver for Transplantation
Author(s) -
FrancoGou R.,
Peralta C.,
MassipSalcedo M.,
Xaus C.,
Serafín A.,
RosellóCatafau J.
Publication year - 2004
Publication title -
american journal of transplantation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.89
H-Index - 188
eISSN - 1600-6143
pISSN - 1600-6135
DOI - 10.1111/j.1600-6143.2004.00532.x
Subject(s) - medicine , liver transplantation , liver regeneration , transplantation , ischemic preconditioning , reperfusion injury , ischemia , oxidative stress , hepatocyte , liver injury , pathology , regeneration (biology) , biology , biochemistry , microbiology and biotechnology , in vitro
The shortage of available organs for liver transplantation has motivated the development of new surgical techniques such as reduced‐size liver transplantation. Ischemia‐reperfusion (I/R) associated with liver transplantation impairs liver regeneration. Ischemic preconditioning is effective against I/R injury in clinical practice of liver tumour resections. The present study evaluated the effect of ischemic preconditioning on reduced‐size liver for transplantation and attempted to identify the underlying protective mechanisms. Hepatic injury and regeneration (transaminases, proliferating cell nuclear antigen [PCNA] labeling index, and hepatocyte growth factor [HGF]) were assessed after reduced‐size orthotopic liver transplantation (ROLT). Energy metabolism, oxidative stress, tumor necrosis factor‐α (TNF) and interleukin‐6 (IL‐6) were examined as possible mechanisms involved in liver regeneration. Ischemic preconditioning reduced transaminase levels and increased HGF levels and the percentage of PCNA‐positive hepatocytes after ROLT. This was associated with a decrease in oxidative stress following ROLT, whereas energy metabolism and hepatic IL‐6 and TNF release were unchanged. The benefits of ischemic preconditioning on hepatic injury and liver regeneration could be mediated, at least partially by nitric oxide. These results suggest a new potential application of ischemic preconditioning in reduced‐size liver transplantation.