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H 1 N 1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines
Author(s) -
KomineAizawa Shihoko,
Suzaki Ai,
Trinh Quang D.,
Izumi Yasuyuki,
Shibata Toshikatsu,
Kuroda Kazumichi,
Hayakawa Satoshi
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2012.01172.x
Subject(s) - virus , trophoblast , virology , cytopathic effect , cell culture , biology , influenza a virus , pandemic , placenta , pregnancy , medicine , fetus , covid-19 , disease , genetics , infectious disease (medical specialty)
Problem Although high maternal mortality was reported in the most recent pandemic of swine‐origin influenza A H 1 N 1/09 ( H 1 N 1/09), its direct effects on the feto‐placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H 1 N 1 influenza A ( H 1 N 1/09) virus. Method of study The H 1 N 1/09 virus was isolated from a patient with influenza, sequenced and identified as the A / N arita/2009 ( H 1 N 1) strain. The trophoblast cell lines Swan71 and HTR 8 were challenged with the virus and examined for the expression of H 1 N 1/09 viral RNA and proteins. Results Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected. Conclusion First trimester human trophoblast cell lines were susceptible to the H 1 N 1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H 1 N 1/09 virus may be limited.