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Ovarian Stimulation Reduces IL ‐6 Release from Mouse and Human Pre‐Implantation Embryos
Author(s) -
Yu Chunhua,
Wang Li,
Li Jianhua,
Guo Chun,
Guo Xinyu,
Zhang Xinyan,
Xu Ye,
Yao Yuanqing
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2012.01142.x
Subject(s) - blastocyst , embryo , zygote , andrology , stimulation , biology , embryo culture , embryogenesis , endocrinology , medicine , microbiology and biotechnology
Problem In our previous study, ovarian stimulation retarded post‐implantation development and caused 92 genes, including interleukin‐6 ( IL ‐6), to be differentially expressed in mouse blastocysts. None of them had been tested at protein level, In this study, we confirm the level change of IL ‐6 m RNA in mouse blastocysts and IL ‐6 protein release to the culture medium from mouse and human pre‐implantation embryos. Method of study IL ‐6 mRNA were measured by PCR and IL ‐6 release to the culture medium were measured by ELISA . Results Superovulation caused a 1.95‐fold reduction in IL‐6 m RNA in mouse blastocysts and reduced IL ‐6 secretion into the culture medium in mouse zygotes which were developed to the blastocyst stage (6.397 ± 0.635 versus 4.799 ± 0.312 pg/mL). Ovarian stimulation also significantly reduced IL‐6 release into the culture medium in human zygotes which were cultured for 2 days (4.713 ± 0.945 versus 4.543 ± 0.796 pg/mL). Conclusion Our findings indicate that IL‐6 might serve as a candidate marker for evaluating embryo developmental competence.