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The Expressions of CD 200 and CD 200 R Molecules on Myeloid and Lymphoid Dendritic Cells in Pre‐Eclampsia and Normal Pregnancy
Author(s) -
DarmochwalKolarz Dorota A.,
KludkaSternik Magdalena,
Chmielewski Tomasz,
Kolarz Bogdan,
Rolinski Jacek,
LeszczynskaGorzelak Bozena,
Oleszczuk Jan
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2012.01126.x
Subject(s) - myeloid , dendritic cell , pregnancy , immunology , medicine , antigen , t cell , eclampsia , immune system , biology , genetics
Problem The purpose of our study was to test the hypothesis that the expressions of CD 200 and CD 200 R tolerance molecules are increased on peripheral blood dendritic cells ( DC s) in normal pregnancy and decreased on peripheral blood DC s in pre‐eclampsia. Method of study Thirty‐three patients with pre‐eclampsia, 38 normal pregnant women, and 10 healthy non‐pregnant women were included in the study. Dendritic cells were isolated from peripheral blood, stained with monoclonal antibodies against blood dendritic cell antigens as well as CD 200 and CD 200 R antigens, and estimated using flow cytometry. Results The expressions of CD 200 and CD 200 R molecules on CD 1c + myeloid and BDCA ‐2 + lymphoid DC s in the first trimester of normal pregnancy were significantly higher when compared to the luteal phase of the ovarian cycle. The expressions of CD 200 molecule on CD 1c + myeloid DC s were significantly lower in the third trimester of normal pregnancy when compared to the second trimester. The expressions of CD 200 R molecule on CD 1c + myeloid DC s and BDCA ‐2 + lymphoid DC s did not differ in pre‐eclampsia and healthy third trimester pregnant women. However, the expressions of CD 200 molecule on CD 1c + myeloid and BDCA ‐2 + lymphoid DC s were significantly higher in pre‐eclampsia when compared to the healthy third trimester pregnant women. Conclusion The results suggest increased tolerogenic properties of myeloid and lymphoid DC s in normal human pregnancy. Moreover, they suggest a decrease in tolerogenic properties of DC s before delivery. It seems possible that higher expressions of CD 200 molecule on CD 1c + myeloid and BDCA ‐2 + lymphoid DC s in pre‐eclampsia may constitute the tolerogenic mechanism secondary to the pro‐inflammatory response that is observed in this syndrome.