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The Mucin MUC 16 ( CA 125) Binds to NK Cells and Monocytes from Peripheral Blood of Women with Healthy Pregnancy and Preeclampsia
Author(s) -
Tyler Chanel,
Kapur Arvinder,
Felder Mildred,
Belisle Jennifer A.,
Trautman Christine,
Gubbels Jennifer A. A.,
Connor Joseph P.,
Patankar Manish S.
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2012.01113.x
Subject(s) - preeclampsia , flow cytometry , immune system , mucin , immunology , receptor , andrology , biology , chemistry , medicine , pregnancy , pathology , genetics
Problem MUC 16 ( CA 125) released from ovarian tumors binds to NK cells and monocytes via the inhibitory receptor S iglec‐9. Here, we investigate whether MUC 16 also binds to circulating immune cells during pregnancy and in women with preeclampsia. Method of study MUC 16 binding was monitored by flow cytometry and immunoprecipitation, and RT ‐ PCR was used to monitor indigenous expression in immune cells. Serum CA 125 levels were measured by a clinical assay. Results MUC 16 was equally distributed on S iglec‐9 pos CD 16 pos / CD 56 dim and CD 16 neg / CD 56 br NK cells in the healthy pregnant and preeclampsia groups. While serum CA 125 levels and number of NK and monocytes were similar, increased binding of MUC 16 was observed on these immune cells in the preeclampsia cohort as compared to the healthy pregnant samples. Conclusion MUC 16 binding to NK cells and monocytes likely contributes to tolerance of the fetal allograft from maternal responses and may also serve as a novel biomarker for preeclampsia.