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Explants of Intact Endometrium to Model Bovine Innate Immunity and Inflammation Ex Vivo
Author(s) -
Borges Álan Maia,
Healey Gareth David,
Sheldon Iain Martin
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2012.01106.x
Subject(s) - ex vivo , endometrium , endometritis , biology , innate immune system , inflammation , andrology , immunology , in vivo , immune system , endocrinology , medicine , pregnancy , genetics , microbiology and biotechnology
Problem Bacterial infections commonly cause bovine endometritis and infertility via innate immune pathways. However, mechanistic studies using isolated cells or chopped tissue may be compromised by the disruption of endometrial architecture and release of damage‐associated molecular patterns. So, this study aimed to establish an ex vivo model of intact bovine endometrium to study innate immunity and inflammation. Method of study Intact bovine endometrium explants were collected using a sterile 8‐mm punch biopsy and cultured ex vivo with bacteria or pathogen‐associated molecules. Interleukin accumulation was measured, and tissue viability was assessed by microscopy, T d T ‐mediated biotin–d UTP nick‐end labelling and lactate dehydrogenase assay. Results Intact endometrium explants accumulated IL ‐6, IL ‐1β and IL ‐8 in response to Gram‐negative or Gram‐positive bacteria, and their purified pathogen‐associated molecules; inflammatory responses were dependent on the stage of oestrous cycle. Explants of intact endometrium maintained viability and tissue architecture, and had lower basal accumulation of interleukins compared with explants using chopped endometrium. Conclusion This study established a tractable ex vivo model of intact endometrium to explore the mechanisms of immunity and inflammation in the bovine endometrium.