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T oll‐Like Receptor Expression and Function in the COV 434 Granulosa Cell Line
Author(s) -
Price Jennifer Claire,
Cronin James,
Sheldon Iain Martin
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2011.01103.x
Subject(s) - chemokine , lipopolysaccharide , immune system , granulosa cell , biology , tlr4 , microbiology and biotechnology , lipoteichoic acid , cell culture , chemistry , follicular phase , immunology , endocrinology , bacteria , genetics , staphylococcus aureus
Problem Bacterial infections perturb ovarian follicle function, despite the lack of immune cells such as macrophages within healthy ovarian follicles. This study examined whether the granulosa cells that line ovarian follicles could, like macrophages, use T oll‐like receptors ( TLR s) to detect pathogen‐associated molecular patterns ( PAMP s) and initiate inflammation. Method of study The COV 434 human granulosa and THP ‐1 macrophage cell lines were used to determine the expression of TLR s and measure the production of cytokines, chemokines and estradiol in response to the PAMP s lipopolysaccharide, peptidoglycan, lipoteichoic acid and flagellin from bacteria. Results The THP ‐1 and granulosa cells expressed mRNA for TLR1‐10 and TLR4‐10 , respectively. The supernatants of THP ‐1 cells accumulated IL ‐1β, IL ‐6, IL ‐8 and CCL 5 in response to PAMP s. Treatment of granulosa cells with PAMP s increased expression of IL1B mRNA after 3 hr, but did not change the accumulation of IL ‐1β, IL ‐6, IL ‐8, CCL 5 or estradiol. Granulosa cells produced IL ‐8 constitutively, and this was reduced using chemical inhibitors for p38 and JNK mitogen‐activated protein kinases. Conclusion The COV 434 human granulosa cell line expresses TLR s and constitutively secretes IL ‐8 but only mounts an inflammatory response to PAMP s at the transcriptional level.