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Rapid and Transient Upregulation of CCL 11 (Eotaxin‐1) in Mouse Ovary During Terminal Stages of Follicular Development
Author(s) -
Kuwabara Yoshimitsu,
Katayama Akira,
Igarashi Tsutomu,
Tomiyama Ryoko,
Piao Hua,
Kaneko Reika,
Abe Takashi,
Mine Katsuya,
Akira Shigeo,
Orimo Hideo,
Takeshita Toshiyuki
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2011.01100.x
Subject(s) - theca , follicular phase , downregulation and upregulation , ovary , ccl11 , human chorionic gonadotropin , endocrinology , biology , medicine , gonadotropin , andrology , eotaxin , chemokine , hormone , receptor , biochemistry , gene
Problem This study aimed to investigate the regulation of expression, localization and physiological role of the CCL 11/ CCR 3 axis in mouse ovary during the periovulatory period. Method of study CCL 11/ CCR 3 expression in the mouse ovary after treatment with pregnant mare serum gonadotropin ( PMSG ) followed by human chorionic gonadotropin (h CG ) 48 hr later was assessed in vivo and in 3‐dimensional cultures in vitro . Results Real‐time RT ‐ PCR analyses revealed transient CCL 11 m RNA upregulation 6 hr after h CG treatment. Immunohistochemical staining of serial ovarian sections demonstrated overlapping expression of CCL 11, CCR 3 and CD 31 endothelial cell marker in the theca‐interstitial layer at 10 hr after h CG treatment. In vitro 3‐dimensional cultures of periovulatory ovarian tissues demonstrated that treatment with anti‐ CCL 11 neutralizing antibody significantly decreased CD 31 transcript. Conclusions Gonadotropin surge leads to transient CCL 11/ CCR 3 axis upregulation in the ovarian theca‐interstitial layer, suggesting that it is involved in periovulatory physiological processes by affecting follicular vessels.