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Multimodality Animal Rotation Imaging System (MARS) for In Vivo Detection of Intraperitoneal Tumors
Author(s) -
Pizzonia John,
Holmberg Jennie,
Orton Sean,
Alvero Ayesha,
Viteri Oscar,
Mclaughlin William,
Feke Gil,
Mor Gil
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2011.01070.x
Subject(s) - in vivo , preclinical imaging , ovarian cancer , pathology , fluorescence lifetime imaging microscopy , intraperitoneal injection , cancer , cancer research , biology , medicine , nuclear medicine , fluorescence , physics , microbiology and biotechnology , quantum mechanics
Citation Pizzonia J, Holmberg J, Orton S, Alvero A, Viteri O, Mclaughlin W, Feke G, Mor G. Multimodality animal rotation imaging system (MARS) for in vivo detection of intraperitoneal tumors. Am J Reprod Immunol 2012; 67: 84–90 Problem Ovarian cancer stem cells (OCSCs) have been postulated as the potential source of recurrence and chemoresistance. Therefore identification of OvCSC and their complete removal is a pivotal stage for the treatment of ovarian cancer. The objective of the following study was to develop a new in vivo imaging model that allows for the detection and monitoring of OCSCs. Method of Study OCSCs were labeled with X‐Sight 761 Nanospheres and injected intra‐peritoneally (i.p.) and sub‐cutaneously (s.c.) to Athymic nude mice. The Carestream In‐Vivo Imaging System FX was used to obtain X‐ray and, concurrently, near‐infrared fluorescence images. Tumor images in the mouse were observed from different angles by automatic rotation of the mouse. Results X‐Sight 761 Nanospheres labeled almost 100% of the cells. No difference on growth rate was observed between labeled and unlabeled cells. Tumors were observed and monitoring revealed strong signaling up to 21 days. Conclusion We describe the use of near‐infrared nanoparticle probes for in vivo imaging of metastatic ovarian cancer models. Visualization of multiple sites around the animals was enhanced with the use of the Carestream Multimodal Animal Rotation System.