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Ectoplacental Cone Induces Resistance to Apoptosis in High Doses of Interferon (IFN)‐γ‐Treated Decidual Cells
Author(s) -
Borbely Alexandre U.,
FonteneleNeto José D.,
Vidsiunas Alex K.,
Gomes Sara Z.,
Hoshida Mara S.,
de Oliveira Sérgio F.,
Bevilacqua Estela
Publication year - 2012
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2011.01060.x
Subject(s) - decidual cells , apoptosis , microbiology and biotechnology , interferon , cone (formal languages) , interferon gamma , decidua , chemistry , immunology , andrology , biology , cytokine , medicine , pregnancy , biochemistry , fetus , genetics , placenta , algorithm , computer science
Citation Borbely AU, Fontenele‐Neto JD, Vidsiunas AK, Gomes SZ, Hoshida MS, de Oliveira SF, Bevilacqua E. Ectoplacental cone induces resistance to apoptosis in high doses of interferon (IFN)‐γ‐treated decidual cells. Am J Reprod Immunol 2012; 67: 73–83 Problem In this study, we explored the relationship between decidual cells (DC) and interferon (IFN)‐γ, in the presence or absence of ectoplacental cone (EC) using a coculture system. Method of study Decidual cells and EC were isolated from pregnant mice on gestation day 7.5. DCs were cultured for 48 hr and then treated with fresh EC. After characterization, they were treated with IFN‐γ, and cell death was evaluated. Results Interferon‐γ drastically increased decidual apoptosis, which was partially reverted by the addition of EC to the IFN‐γ‐treated decidual culture. Moreover, the addition of EC to non‐treated DC cultures was also capable of attenuating death rates. Conclusion Resistance to apoptosis may be induced in DC by the EC. This suggests that EC may participate in the inhibition of IFN‐γ‐dependent apoptosis and, therefore, play important role for DC survival in a cytokine‐enriched placental environment.