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Relevance of Syndecan‐1 in the Trophoblastic BeWo Cell Syncytialization
Author(s) -
Prakash Golla Jaya,
Suman Pankaj,
Gupta Satish Kumar
Publication year - 2011
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2011.01017.x
Subject(s) - forskolin , syncytiotrophoblast , cell fusion , syndecan 1 , syncytium , cytotrophoblast , microbiology and biotechnology , biology , trophoblast , endocrinology , medicine , chemistry , cell , biochemistry , placenta , fetus , pregnancy , genetics , stimulation
Citation Prakash GJ, Suman P, Gupta SK. Relevance of syndecan‐1 in the trophoblastic BeWo cell syncytialization. Am J Reprod Immunol 2011; 66: 385–393 Problem To investigate the role of syndecan‐1 in the differentiation of the BeWo cells into syncytiotrophoblast. Method of study BeWo cells were stimulated with forskolin to form syncytia, and the expression of syndecan‐1, desmoplakin I+II, human chorionic gonadotrophin (hCG) and angiogenesis‐associated factors was analyzed. Syndecan‐1 was silenced by siRNA to evaluate its involvement in the forskolin‐mediated syncytia formation. Results Treatment of the BeWo cells with forskolin led to a significant increase in the syncytia formation. It was associated with an increase in the expression of syndecan‐1 with a concomitant decrease in the expression of desmoplakin I+II. Forskolin treatment of the BeWo cells also led to an increase in the secretion of soluble endoglin, whereas no change was observed in the soluble fms‐like tyrosine kinase‐1. Silencing of the syndecan‐1 expression in BeWo cells led to a significant decrease in cell fusion both in the presence and in the absence of forskolin. It was associated with a significant decrease in hCG level in the conditioned medium. Conclusion Syndecan‐1 is up‐regulated in BeWo cells during differentiation and its silencing inhibits syncytialization and thus could be a useful biomarker for syncytiotrophoblast formation.