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Is Apolipoprotien E codon 112 Polymorphisms Associated with Recurrent Pregnancy Loss?
Author(s) -
Ozornek Hakan,
Ergin Elif,
Jeyendran Rajasingam S.,
Ozay Alev T.,
Pillai Delanie,
Coulam Carolyn
Publication year - 2010
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2010.00814.x
Subject(s) - pregnancy , genotype , point mutation , cytosine , buccal swab , medicine , gynecology , biology , genetics , obstetrics , mutation , gene
Citation Ozornek H, Ergin E, Jeyendran RS, Ozay AT, Pillai D, Coulam C. Is Apolipoprotien E codon 112 polymorphisms associated with recurrent pregnancy loss? Am J Reprod Immunol 2010; 64: 87–92 Problem To compare the prevalence of 112T>C point mutations among women experiencing RPL with fertile control women. Method of Study Buccal swabs were obtained from 232 individuals: 136 with a history of ≥2 abortions, 37 with at least 2 live births and 59 with a history of deep vein thrombosis (DVT). DNA was extracted and PCR amplification of Apo E codons was performed. Results The allelic frequency of a cytosine at position 112 was 11.4% (31/272) among patients experiencing RPL, compared with a frequency of 5.4% (4/74) among the fertile controls ( P = 0.19) and 19.5% (23/118) among individuals with a history of DVT. However, significantly more E3/E4 and E4/E4 genotypes were seen among individuals experiencing RPL and DVT than fertile controls ( P < 0.05). Conclusion Apo E4 codon 112C point mutation is, by itself, not associated with an elevated risk of recurrent pregnancy loss, but rather codon 112C in association with codon 158C is a risk factor for RPL.