Premium
SHORT COMMUNICATION: Increased Expression of Glutathione by Estradiol, Tumor Necrosis Factor‐Alpha, and Interleukin 1‐Beta in Endometrial Stromal Cells
Author(s) -
Lee Sa Ra,
Kim Sung Hoon,
Lee Hoi Woul,
Kim YoungHoon,
Chae Hee Dong,
Kim ChungHoon,
Kang Byung Moon
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00760.x
Subject(s) - glutathione , proinflammatory cytokine , stromal cell , tumor necrosis factor alpha , endocrinology , medicine , chemistry , peritoneal cavity , andrology , inflammation , biochemistry , enzyme , surgery
Problem The intracellular antioxidant system, based on glutathione (GSH), plays a key role in endometrial detoxification reactions and has been proposed to be involved in the pathogenesis endometriosis. This study was designed to evaluate whether estradiol (E 2 ) and proinflammatory cytokines have any effects on expression of glutathione in endometrial stromal cells (ESCs). Method of study Glutathione levels were measured utilizing high‐performance liquid chromatography following in vitro culture and treatment of ESCs with estradiol, tumor necrosis factor‐alpha (TNF‐α) and interleukin 1‐beta (IL‐1β). Results The GSH level in E 2 (10 −8 m ) treatment group was significantly higher than in the control group at 48 h ( P < 0.05). In vitro treatment of ESCs with TNF‐α 10 ng/mL as well as E 2 (10 −8 m ) plus TNF‐α 10 ng/mL for 48 hr also led to a significant increase in GSH level ( P < 0.05; P < 0.05, respectively). Both IL‐1β 10 ng/mL and E 2 (10 −8 m ) plus IL‐1β 10 ng/mL for 48 hr increased GSH level significantly ( P < 0.05; P < 0.05, respectively) as well. Conclusions These findings might suggest that increased production of estradiol and proinflammatory cytokines in the peritoneal cavity possibly leads to the establishment of endometriosis through increased level of GSH.