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ORIGINAL ARTICLE: Multiple Cytokine Profile in Plasma and Amniotic Fluid in a Mouse Model of Pre‐Term Labor
Author(s) -
Yang Qing,
ElSayed Yasser,
RosenbergHasson Yael,
Hirschberg David L.,
Nayak Nihar R.,
Schilling James,
Madan Ashima
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00743.x
Subject(s) - cytokine , inflammation , chemokine , amniotic fluid , immunology , proinflammatory cytokine , medicine , endocrinology , andrology , pregnancy , biology , fetus , genetics
Problem The rate of pre‐term birth in the United States continues to rise despite several interventions. Induction of pro‐inflammatory cytokines and chemokines has been implicated in the activation of the cascade of events resulting in pre‐term labor. To date, no comprehensive panel of the cytokine profile in PTL has been published. Method of study To address cytokine profiles in pre‐term labor, levels of 19 plasma and amniotic fluid cytokines were measured using a multiplex immunoassay in an inflammation‐induced murine model of pre‐term labor. Results Pro‐inflammatory mediators, RANTES, KC, IL‐6, and IL‐12p40 were increased by 3 hr and remained high at 15 hr. Concentrations of KC, IL‐6, IL‐1β, and MIP‐1α were increased in the amniotic fluid at 15 hr. Plasma levels of anti‐inflammatory mediators IL‐10 and IL‐13 at 15 hr were unchanged and decreased respectively. Conclusion These results suggest that stimulation of several pro‐inflammatory cytokines occurs very early in the cascade of events and remains increased, whereas anti‐inflammatory cytokines are either unchanged or decreased until the onset of delivery in an inflammation‐induced mouse model of pre‐term labor.