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ORIGINAL ARTICLE: Effects of Peritoneal Fluid from Endometriosis Patients on Interferon‐γ‐Induced Protein‐10 (CXCL10) and Interleukin‐8 (CXCL8) Released by Neutrophils and CD4 + T Cells
Author(s) -
Kim JiYeon,
Lee DongHyung,
Joo JongKil,
Jin JunO,
Wang JiWon,
Hong YoungSeoub,
Kwak JongYoung,
Lee KyuSup
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00722.x
Subject(s) - chemokine , peritoneal fluid , cxcl10 , interleukin 8 , endometriosis , interleukin , immunology , cytokine , chemistry , monocyte , interferon , pathogenesis , inflammation , biology , medicine
Problem Intraperitoneal immuno‐inflammatory changes may be associated with the pathogenesis of endometriosis. We evaluated the effects of peritoneal fluid obtained from patients with endometriosis (ePF) on the release of interferon‐γ‐induced protein‐10 (IP‐10/CXCL10) and interleukin‐8 (IL‐8/CXCL8) by neutrophils, CD4 + T cells, and monocytes. Method of study Neutrophils, CD4 + T cells, and monocytes were cultured with ePF and the chemokine levels in the supernatants were then measured using enzyme‐linked immunosorbent assay. Results The addition of ePF to cultures of CD4 + T cells led to a significant increase in the release of IP‐10 when compared with control PF without endometriosis (cPF). There was a positive correlation between the levels of IL‐8 and IP‐10 in ePF ( R = 0.89, P = 0.041), but not between the levels of IP‐10 and IL‐8 released by neutrophils, CD4 + T cells, and monocytes. The levels of IP‐10 in ePF were positively correlated with the release of IP‐10 by ePF‐treated neutrophils ( R = 0.89, P < 0.001), CD4 + T cells ( R = 0.93, P < 0.001), and monocytes ( R = 0.70, P = 0.01). Moreover, the addition of ePF significantly enhanced the interferon‐γ‐induced release of IP‐10 by nuetrophils and CD4 + T cells. Conclusion These findings suggest that neutrophils and T cells release differential levels of IP‐10 and IL‐8 in response to stimulation with ePF, and that these cells are a major source of IP‐10 in the PF of endometriosis patients.