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ORIGINAL ARTICLE: Two Different Homing Pathways Involving Integrin β7 and E‐selectin Significantly Influence Trafficking of CD4 Cells to the Genital Tract Following Chlamydia muridarum Infection
Author(s) -
Kelly Kathleen A.,
Chan Ann M.,
Butch Anthony,
Darville Toni
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00704.x
Subject(s) - chlamydia , homing (biology) , immunology , biology , chlamydia trachomatis , integrin , population , l selectin , in vivo , microbiology and biotechnology , medicine , cell adhesion molecule , cell , ecology , genetics , environmental health
Problem Chlamydia trachomatis causes STI and reproductive dysfunction worldwide which is not preventable with antibiotics. Identifying a population of endocervical T cells to target in vaccine development would enhance efficacy. Method of study Trafficking of murine CD4+ lymphocytes to Chlamydia muridarum infected genital tract (GT) tissue in vivo was measured using adoptive transfer studies of fluorescent CD4+ T cells from integrin β7−/− mice or mice which lack E‐selectin on endothelial cells. Results Murine in vivo migration studies showed that lack of α4β7 or E‐selectin significantly reduced trafficking of CD4 T cells to the GT of mice infected with C. muridarum . Conclusion CD4+ T cells use at least two different adhesive mechanisms involving an integrin of the mucosal homing pathway and selectin pathway to accumulate in the GT during C . muridarum infection.