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ORIGINAL ARTICLE: Leukocyte Activation and Circulating Leukocyte‐Derived Microparticles in Preeclampsia
Author(s) -
Lok Christianne A.R.,
Jebbink Jiska,
Nieuwland Rienk,
Faas Marijke M.,
Boer Kees,
Sturk Augueste,
Van Der Post Joris A.M.
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00701.x
Subject(s) - preeclampsia , tumor necrosis factor alpha , monocyte , inflammation , immunology , elastase , endocrinology , cytotoxic t cell , flow cytometry , medicine , andrology , biology , pregnancy , in vitro , biochemistry , genetics , enzyme
Problem Preeclampsia shows characteristics of an inflammatory disease including leukocyte activation. Analyses of leukocyte‐derived microparticles (MP) and mRNA expression of inflammation‐related genes in leukocytes may establish which subgroups of leukocytes contribute to the development of preeclampsia. Method of Study Blood samples were obtained from preeclamptic patients, normotensive pregnant and non‐pregnant controls. sL‐selectin and elastase were measured by ELISA. mRNA was isolated from leukocytes and gene expression was determined by multiplex ligation‐dependent probe amplification (MLPA). MP were characterized by flow cytometry. Results Altered concentrations of sL‐selectin and elastase confirmed leukocyte activation in preeclampsia. These leukocytes showed up‐regulation of Nuclear Factor of Kappa light chain gene enhancer in B Cells inhibitor (NFκB‐1A) and cyclin‐dependent kinase inhibitor (CDKN)‐1A compared with normotensive pregnant women. interleukin‐1 Receptor Antagonist (IL‐1RA) and tumor necrosis factor (TNF)‐R1 were increased compared with those in non‐pregnant controls. Monocyte‐derived MP were elevated in preeclamptic patients compared with pregnant women. The numbers of cytotoxic T‐cell‐derived and granulocyte‐derived MP were elevated compared with those of non‐pregnant women. Conclusion Leukocytes are activated in preeclampsia. A pro‐inflammatory gene expression profile is not prominent, although differences in mRNA expression can be detected. Increased levels of particular subsets of leukocyte‐derived MP reflect activation of their parental cells in preeclampsia.