Premium
ORIGINAL ARTICLE: Functional Changes of Human Peripheral B‐Lymphocytes in Pre‐Eclampsia
Author(s) -
Liao AiHua,
Liu LiPing,
Ding WenPing,
Zhang Ling
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00697.x
Subject(s) - peripheral blood mononuclear cell , pokeweed mitogen , cd38 , antibody , monoclonal antibody , endocrinology , andrology , medicine , immunology , microbiology and biotechnology , biology , chemistry , in vitro , stem cell , biochemistry , genetics , cd34
Problem The aim of our study was to investigate the functional changes of human peripheral B‐lymphocytes in healthy and pre‐eclamptic pregnancies. Method of study Twenty patients with pre‐eclampsia and 15 healthy third‐trimester pregnant women were recruited in this study. Peripheral blood mononuclear cells (PBMCs) were isolated and directly stained with fluorescein isothiocyanate (FITC)‐labeled anti‐CD27 monoclonal antibody (mAb) and phycoerythrin (PE)‐labeled anti‐CD38 mAb. The percentages of the individual B‐cell subsets were estimated out of total lymphocytes by flow cytometric analysis. Additionally, the enriched PBMCs were cultured with or without the stimulation of pokeweed mitogen (PWM) for 5 days. Then morphologic observation of plasma cells was analysed by Wright‐Giemsa stain, and antibody‐producing cells were detected by enzyme‐linked immunospot assay. Results The percentage of CD27 − CD38 − naïve B‐cells and CD27 − CD38 + plasma cells did not differ between study groups ( P > 0.05). The percentage of CD27 + CD38 − memory B‐cells and CD27 + CD38 + plasma cell pre‐cursors increased in pre‐eclamptic women compared with the controls ( P < 0.05). Irrespective of whether the PBMCs were stimulated with or w/o PWM in vitro , the mean percentages of generated plasma cells were significantly higher in pre‐eclamptic group than in the controls ( P < 0.05). There were more antibody‐producing cells in pre‐eclamptic women following the activation of PWM than those in the controls ( P < 0.01). Conclusion Our findings implicate that the functional changes of human circulating B‐cells might contribute to the etiology of pre‐eclampsia.