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ORIGINAL ARTICLE: TNFα Gene Silencing Reduced Lipopolysaccharide‐Promoted Proliferation of Endometriotic Stromal Cells
Author(s) -
Miyamoto Ayako,
Taniguchi Fuminori,
Tagashira Yukiko,
Watanabe Ayako,
Harada Tasuku,
Terakawa Naoki
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2009.00691.x
Subject(s) - stromal cell , gene silencing , tumor necrosis factor alpha , transfection , cancer research , lipopolysaccharide , biology , microbiology and biotechnology , chemistry , cell culture , gene , immunology , genetics
Problem We previously reported that lipopolysaccharide (LPS)‐promoted endometriotic stromal cell (ESC) proliferation by inducing TNFα production. The aim of this study was to investigate the efficacy of TNFα gene silencing on LPS‐treated ESCs. Method of study Endometriotic stromal cells (ESCs) and endometrial stromal cells (ESCs) (EMSCs) were obtained from ovarian chocolate cysts and uterine myoma, respectively. Using PCR array, LPS‐induced gene expression profiling after transfection of TNFα siRNA into ESCs was performed. Down‐regulated genes by TNFα silencing were examined using real‐time RT‐PCR. Effect of TNFα silencing was examined using ELISA and BrdU incorporation, respectively. Results In PCR array, TNFα silencing in ESCs repressed LPS‐induced expression of cIAP2 and IL‐8, NFκB pathway responsive genes. After adding LPS, the levels of cIAP2 and IL‐8 expression in ESCs were higher compared with those in EMSCs. TNFα silencing attenuated the LPS‐induced ESC proliferation. Conclusion Tumor necrosis factor α may be involved in cell proliferation of endometriotic tissues.