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ORIGINAL ARTICLE: Murine Monoclonal Antibody 26 Raised Against Tetanus Toxoid Cross‐Reacts with β 2 ‐Glycoprotein I: Its Characteristics and Role in Molecular Mimicry
Author(s) -
InicKanada Aleksandra,
Stojanovic Marijana,
Zivkovic Irena,
Kosec Dusko,
Micic Mileva,
Petrusic Vladimir,
ZivancevicSimonovic Snezana,
Dimitrijevic Ljiljana
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2008.00660.x
Subject(s) - molecular mimicry , epitope , monoclonal antibody , toxoid , antibody , polyclonal antibodies , glycoprotein , microbiology and biotechnology , in vitro , biology , monoclonal , chemistry , immunology , biochemistry , immunization
Problem Studies on experimental antiphospholipid syndrome (APS) models proved that molecular mimicry between plasma protein β 2 ‐glycoprotein I (β 2 GPI) and structure within micro‐organisms or their products, might be a cause for experimental APS. Considering the heterogeneity of polyclonal antiphospholipid antibodies (aPLs), it is important to define the precise characteristics of pathogenic aPLs. To avoid the influence of polyclonality and to further analyse the connection between molecular mimicry and APS, we produced monoclonal antibodies (MAbs) against tetanus toxoid (TTd) and tested their reactivity against β 2 GPI. Method of study In this report, we analysed the characteristics of MAb26 raised against TTd and cross‐reactive with β 2 GPI: its binding properties in various in vitro immunoassays, its specific interactions with surface epitopes expressed on apoptotic cells and its role in vivo . Results We have demonstrated that MAb26: (i) binds β 2 GPI being immobilized on an appropriate surface: irradiated polystyrene plates, non‐irradiated plates pre‐coated with anionic phospholipids and polyvinylidene fluoride membrane; (ii) binds specifically to apoptotic but not to viable cells and the binding is β 2 GPI‐dependent; and (iii) induces a pathologic pregnancy outcome when passively injected into BALB/c mice. Conclusion This study concluded that certain subpopulations of antibodies raised against TTd and cross‐reactive with β 2 GPI, because of the molecular mimicry mechanism, could have pathologic potential.