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ORIGINAL ARTICLE: Genistein Reverses Diminished T‐Cell Signal Transduction, Induced by Post‐Menopausal Estrogen Levels
Author(s) -
Parry J. Preston,
Taylor Douglas D.,
Nakajima Steven T.,
GercelTaylor Cicek
Publication year - 2009
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2008.00658.x
Subject(s) - genistein , estrogen , endocrinology , medicine , signal transduction , jurkat cells , messenger rna , reverse transcription polymerase chain reaction , gene expression , chemistry , biology , t cell , gene , microbiology and biotechnology , biochemistry , immunology , immune system
Problem This study addressed the ability of genistein to reverse the loss of T‐cell signaling components, induced by estrogen deficiency associated with aging. Method of study Using Jurkat 6.1 T cells, genistein regulation of CD3ζ, JAK3, and NFκB was analysed by Western immunoblotting at 4 or 40 pg/mL (post‐ and pre‐menopausal levels, respectively) estradiol (E 2 ). Corresponding gene expressions were quantified by real‐time polymerase chain reaction (RT‐PCR). For functionality, levels of IL‐2 were correlated with its transcription by RT‐PCR. Results At 4 pg/mL E 2 , signaling proteins were decreased compared with 40 pg/mL: CD3ζ, 1.58‐fold; JAK3, 1.75‐fold; and NFκB, 1.73‐fold ( P < 0.001). Genistein, at 0.5 and 5.0 μ m , added to 4 pg/mL E 2 induced their expression to 40 pg/mL levels. While significantly diminished IL‐2 mRNA levels were observed at 4 pg/mL E 2 , genistein induced IL‐2 mRNA to 40 pg/mL levels. Conclusion Genistein restores CD3ζ, NFκB and JAK3 proteins and mRNAs at post‐meonopausal estrogen levels, in vitro , with no significant effect at pre‐menopausal estrogen levels.