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ORIGINAL ARTICLE: Development of Human Graafian Follicles Following Transplantation of Human Ovarian Tissue into NOD/SCID/γcnull Mice
Author(s) -
Terada Yukihiro,
TerunumaSato Yumi,
KakoiYoshimoto Tomoko,
Hasegawa Hisataka,
Ugajin Tomohisa,
Koyanagi Yoshio,
Ito Mamoru,
Murakami Takashi,
Sasano Hironobu,
Yaegashi Nobuo,
Okamura Kunihiro
Publication year - 2008
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2008.00653.x
Subject(s) - ovarian cortex , transplantation , antral follicle , follicular phase , ovary , ovarian follicle , biology , cortex (anatomy) , folliculogenesis , immunohistochemistry , ovarian tissue cryopreservation , andrology , endocrinology , medicine , ovarian tissue , immunology , embryo , cryopreservation , fertility preservation , microbiology and biotechnology , fertility , population , environmental health , neuroscience
Problem Transplantation of human ovarian cortex into host mice may permit various kinds of challenges in reproductive medicine. A novel immunodeficient mouse strain (NOD/SCID/γcnull: NOG) has been developed as a host of transplantation of human tissue. Method of study Human ovarian cortex was transplanted into various sites of NOG mice and human follicular development was examined by immunohistochemistry. Results Transplantation of human ovarian tissue into NOG mice resulted in approximately similar tissue survival and follicle growth as did transplantation into non‐obese diabetic‐severe combined immunodeficient mice. The human Graafian follicule from NOG mouse expressed the same steroidogenic enzymes as observed in human Graafian follicles, which developed in the human body. The NOG mice’s ovarian bursa was better placed for transplantation than the back skin or kidney capsule. Conclusion These results represent the successful generation and biological confirmation of the human Graafian follicles from the human ovarian cortex in the NOG mice.