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The Investigation on the Value of Repeat and Combination Test of ACA and Anti‐β 2 ‐GPI Antibody in Women with Recurrent Spontaneous Abortion
Author(s) -
Bao Shi Hua,
Wang Xi Peng,
Lin Qi De,
Di Wen,
Xu Liang,
Ding Chuan Wei
Publication year - 2008
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2008.00638.x
Subject(s) - abortion , antibody , value (mathematics) , medicine , test (biology) , gynecology , obstetrics , andrology , immunology , pregnancy , biology , mathematics , genetics , statistics , paleontology
Problem In order to investigate the value of anticardiolipin antibodies (ACA) and anti‐β 2 ‐GPI antibodies detection in screening autoimmune type recurrent spontaneous abortion and its clinic application in antiphospholipid syndrome diagnosis, we adopt repeat combined ACA and anti‐β 2 ‐GPI antibodies detection in this study. Method of study Sera were collected from patients and work‐up was done for detection of ACA and anti‐β 2 ‐GPI antibodies by enzyme‐linked immunosorbent assay (ELISA). The work‐up was done for detection of antibodies once in every 6 weeks for 14 times consecutively. Results The repeated and combined detection of ACA and anti‐β 2 ‐GPI antibodies detection could raise the positivity rate up to 21.8% ( P < 0.05) in comparison with positive for ACA alone (14.1%), positive for anti‐β 2 ‐GPI alone (3.1%), and concurrently positive for both ACA and anti‐β 2 ‐GPI antibodies (4.6%). In 91 confirmed positive antiphospholipid antibodies (APA) patients, with more frequent screening for ACA and anti‐β 2 ‐GPI antibodies, more patients with APA were found. The positive rate of five and more screenings was over 81.32%, which was statistically significant ( P < 0.05), in comparison with that of four or less screenings (68.13%). Conclusion Our data implied that it would be appropriate to take over five or more screenings of combined ACA and anti‐β 2 ‐GPI antibodies detection in suspect patients to facilitate the positive diagnostic rate for autoimmune type RSA.