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ABSTRACTS: 5
In vivo gene delivery of the murine uterus: what and why?
Author(s) -
Rodde N,
Munaut C,
Prince S,
Olivier F,
Bellemin AL,
Chaouat G,
Sandra O
Publication year - 2008
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2008.00626_5.x
Subject(s) - transfection , in vivo , uterus , gene delivery , transgene , gene , genetic enhancement , luciferase , biology , plasmid , microbiology and biotechnology , reporter gene , gene expression , andrology , medicine , endocrinology , genetics
Problem: The in vivo gene delivery offers an alternative for affecting the expression of genes without deriving transgenic animals. In order to investigate the biological functions of genes during implantation, the in vivo transfection of the murine uterus was assessed using a non‐cationic agent. Material and Methods: Various routes of administration were tested using complexes of JetPEI (Polyplus‐Transfection) and pEGFPluc plasmid injected into mice. The luciferase activity was quantified and the GFP cell localisation was investigated in the uterus. The consequences of the injections on the pregnancy were estimated. Results: The efficiency of the in vivo transfection in the uterus as well the type of transfected cells depends on the route of injection. No apparent effect was seen on the pregnancy outcome. Conclusion: Investigating the function of a gene during implantation in the mouse appears feasible using the jetPEI‐based method ofgene delivery. Targeted analyses of specific genes are in progress.