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Increased Natural Killer Cell Cytotoxicity and IL‐2 Production in Recurrent Spontaneous Abortion
Author(s) -
Hadinedoushan Hossein,
Mirahmadian Mahroo,
Aflatounian Abbas
Publication year - 2007
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2007.00524.x
Subject(s) - cytotoxicity , peripheral blood mononuclear cell , natural killer cell , immunology , flow cytometry , biology , cytokine , andrology , abortion , medicine , in vitro , biochemistry , pregnancy , genetics
Problem To determine whether natural killer (NK) cells cytotoxicity in peripheral blood is altered in patients with a history of recurrent spontaneous abortion (RSA); also, if there is any correlation between cytokine production and NK cytotoxicity. Method of study In this case–control study, 21 patients with RSA within 24 hr of the last abortion (group I), and 32 pregnants with no history of abortion (group II) were surveyed. NK cell cytotoxicity was evaluated by flow cytometry, and IL‐2, IL‐10, transforming growth factor β1 were measured in cell culture supernatant by ELISA method. Results Group I showed higher NK cytotoxicity than group II at all of effector to target (E:T) ratios ( P ≤ 0.045).The correlation between production of IL‐2 and NK cytotoxicity was positively significant ( R = 0.350, P = 0.001). Group I had significantly higher levels of IL‐2 than group II ( P = 0.001). In group II, the production of IL‐10 by peripheral blood mononuclear cells was higher than group I ( P = 0.002). Conclusion Increased NK cell cytotoxicity and high level of IL‐2 may be considered as a risk factor for RSA.