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Redirection of Cytokine Production By Lymphocytes From Women with Pre‐Term Delivery By Dydrogesterone
Author(s) -
Raghupathy Raj,
Al Mutawa Esraa,
Makhseed Ma'asoumah,
AlAzemi Majedah,
Azizieh Fawaz
Publication year - 2007
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2007.00488.x
Subject(s) - dydrogesterone , cytokine , peripheral blood mononuclear cell , medicine , tumor necrosis factor alpha , immunology , interleukin 10 , interferon gamma , interleukin , endocrinology , biology , in vitro , estrogen , biochemistry
Problem To study the ability of dydrogesterone to modulate the production of pro‐inflammatory and anti‐inflammatory cytokines by lymphocytes from women undergoing pre‐term delivery (PTD). Method of study Peripheral blood mononuclear cells (PBMC) from 18 subjects undergoing PTD were stimulated with the mitogen phytohemagglutinin in the presence and absence of progesterone and dydrogesterone. The levels of interferon (IFN)‐ γ , tumor necrosis factor (TNF)‐ α , interleukin (IL)‐4, and IL‐10 in culture supernatants were then estimated by enzyme‐linked immunoabsorbant assay. Cytokine production in the presence and absence of progesterone and dydrogesterone were compared. Results The exposure of PBMC to dydrogesterone resulted in a significant inhibition in the production of the pro‐inflammatory cytokines IFN‐ γ and TNF‐ α and a significant increase in the levels of the anti‐inflammatory cytokine IL‐4, resulting in a substantial shift in the ratio of Th1/Th2 cytokines. Conclusion Dydrogesterone induces a shift in cytokine bias, by inhibiting pro‐inflammatory cytokine production and increasing anti‐inflammatory cytokine production.