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Establishment of the Porcine Epitheliochorial Placenta Is Associated with Endometrial T‐Cell Recruitment
Author(s) -
Dimova Tanya,
Mihaylova Anastassia,
Spassova Petia,
Georgieva Rayna
Publication year - 2007
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2007.00472.x
Subject(s) - endometrium , placenta , flow cytometry , andrology , cytotoxic t cell , biology , uterus , in situ , immunohistochemistry , peripheral blood , medicine , pregnancy , endocrinology , chemistry , immunology , fetus , in vitro , biochemistry , genetics , organic chemistry
Problem We assessed implantation‐associated quantitative changes in peripheral blood and endometrial T lymphocytes throughout epitheliochorial placenta formation. Method of study T‐cell subsets were investigated in 10‐, 15‐, 20‐, 30‐, and 40‐day pregnant and non‐pregnant sows by flow cytometry and immunohistochemistry. Results Endometrial total T, T cytotoxic (Tc), and T helper (Th) cells were in peak numbers at the attachment phase of implantation and Tc cells persisted in high proportions up to placental establishment. The number of γ δ T lymphocytes was relatively small and implantation‐independent. In situ , T cells increased in number with the advancement of implantation and formed T‐cell clusters with implantation phase‐dependent location. Percentages of peripheral blood T cells were not significantly changed throughout the implantation. Conclusion Superficial and adeciduate implantation of pigs has a profound effect on the number of total T, Tc, and Th cells and pattern of distribution of endometrial T cells in situ .