Premium
Granulated and Non‐Granulated Decidual Prolactin‐Related Protein‐Positive Decidual Cells in the Pregnant Mouse Endometrium
Author(s) -
Candeloro Luciane,
Zorn Telma M.T.
Publication year - 2007
Publication title -
american journal of reproductive immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.071
H-Index - 97
eISSN - 1600-0897
pISSN - 1046-7408
DOI - 10.1111/j.1600-0897.2006.00452.x
Subject(s) - decidual cells , decidua , paracrine signalling , endometrium , perlecan , uterus , biology , population , prolactin , immunocytochemistry , microbiology and biotechnology , andrology , medicine , endocrinology , extracellular matrix , pregnancy , proteoglycan , placenta , hormone , fetus , receptor , biochemistry , genetics , environmental health
Problem Identification of the cell types responsible for the synthesis of decidual prolactin‐related protein (dPRP) in the pregnant mouse endometrium. Method of study Histochemistry and immunocytochemistry were used to determine peri‐implantation dPRP and perlecan distribution in the mouse uterus. Results We identified dPRP in pre‐decidual and mature decidual cells from days 5 to 12 of pregnancy. On day 8, dPRP immunoreactivity was detected within cytoplasmic granules of a specific population of granulated decidual cells (GDCs). In mesometrial decidual cells, weak immunoreactivity was seen from days 7 to 14. Between days 11 and 14, dPRP was found in cytoplasm and in the extracellular matrix surrounding islands of spongiotrophoblast. Perlecan, a heparan sulfate proteoglycan, was co‐localized with dPRP. Conclusion GDCs are a putative source of dPRP in pregnant mice. Co‐localization of perlecan with dPRP suggests that the former acts as a dPRP reservoir and facilitates its paracrine effect in developing placental tissues.